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Avaliação do potencial fisiológico de sementes de pepino utilizando sistema computadorizado de análise de imagens de plântulas (SVIS®)

机译:使用计算机幼苗图像分析系统(SVIS®)评估黄瓜种子的生理潜力

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摘要

The software Seed Vigor Imaging System (SVIS®), has been successfully used to evaluate seed physiological potential by automated analyses of scanned seedlings. In this research, the efficiency of this system was compared to other tests accepted for assessing cucumber (Cucumis sativus L.) seed vigor of distinct seed lots of Supremo and Safira cultivars. Seeds were subjected to germination, traditional and saturated salt accelerated aging, seedling emergence, seedling length and SVIS analyses (determination of vigor indices and seedling growth uniformity, lengths of primary root, hypocotyl and whole seedlings). It was also determined whether the definition of seedling growth/uniformity ratios affects the sensitivity of the SVIS®. Results showed that analyses SVIS have provided consistent identification of seed lots performance, and have produced information comparable to those from recommended seed vigor tests, thus demonstrating a suitable sensitivity for a rapid and objective evaluation of physiological potential of cucumber seeds. Analyses of four-days-old cucumber seedlings using the SVIS® are more accurate and growth/uniformity does not affect the precision of results.
机译:种子活力成像系统(SVIS®)软件已通过自​​动分析扫描的幼苗成功地用于评估种子的生理潜力。在这项研究中,将该系统的效率与评估Supremo和Safira品种的不同种子批次的黄瓜(Cucumis sativus L.)种子活力所接受的其他测试进行了比较。对种子进行发芽,传统盐和饱和盐加速老化,幼苗出苗,幼苗长度和SVIS分析(确定活力指数和幼苗生长均匀性,初生根,下胚轴和整个幼苗的长度)。还确定了幼苗生长/均匀度比的定义是否影响SVIS®的敏感性。结果表明,分析SVIS提供了对种子批次性能的一致鉴定,并产生了与推荐的种子活力测试相当的信息,从而证明了用于快速客观地评估黄瓜种子生理潜力的合适敏感性。使用SVIS®对四天龄黄瓜幼苗的分析更加准确,并且生长/均匀性不会影响结果的准确性。

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